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Jackson Immuno westgrove
Westgrove, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/westgrove/Alexa+Fluor+488+AffiniPure+Goat+Anti-Rabbit+IgG/pm41746734-222-58-55
Average 96 stars, based on 1504 article reviews

westgrove - by Bioz Stars, 2026-09

96/100 stars

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Labeling:

Article Title: Disrupting the transmembrane domain interface between PMP22 and MPZ causes peripheral neuropathy.
Article Snippet: .. Reagent or Resource Source Identifier Antibodies mouse α-GFP monoclonal Santa Cruz Technology, Santa Cruz, CA sc-9996 mouse α-HA monoclonal BioLegend, San Diego, CA cat# 901514 mouse α-myc-Tag (9B11) monoclonal Cell Signaling Technology, Danvers, MA cat# 2276 rabbit α-mCherry polyclonal EMD Millipore Corp., Burlington, MA AB356482 Peroxidase-conjugated (HRP) α- mouse Cell Signaling Technology cat#:70776S α-rabbit secondary antibody Cell Signaling Technology cat# 7074S α-GFP nanobody agarose Uiowa Protein crystallography facility Chicken α-GFP for immunogold labeling Abcam, Waltham, MA cat# ab13970 Rabbit α-mCherry for immunogold labeling Rockland Immunochemicals, Limerick PA cat#600-401-379 6 nm gold secondary donkey α- rabbit Jackson Immunoresearch, WestGrove, PA cat# 711-195-152 12 nm gold secondary donkey α- chicken Jackson Immunoresearch, WestGrove, PA cat# 703-205-155 Reagent or Chemical Dodecyl-β-D-maltoside (DDM) Anatrace (Maumee, OH) D310S cholesteryl hemisuccinate (CHS) Anatrace (Maumee, OH) CH210 Protease inhibitors cOmplete lacking EDTA Sigma (St. Louis, MO) Product COEDTAF-RO Pefabloc Sigma (St. Louis, MO) Product 11873601001 Dulbecco’s modified Eagle’s medium (DMEM) Gibco, Grand Island, NY REF: 11965-092 10% fetal bovine serum (FBS) Gibco, Grand Island, NY REF: 26140079 16% EM grade paraformaldehyde solution Electron Microscopy Sciences/ Fisher scientific REF: EMS 15710 Microscope sample carrier Leica, Wetzlar, Germany cat# 16770134 Dome-type carrier Leica, Wetzlar, Germany Software Fiji software (Image J) https://imagej.net/software/fiji/downloads Graphpad Prism https://www.graphpad.com/features FlowJo v10 https://www.flowjo.com T-coffee https://tcoffee.crg.eu/apps/tcoffee/index.html Photoshop https://www.adobe.com CS6 LAS X Microlab Leica, Wetzlar, Germany TIA software – Formerly Field Electron and Ion Company, FEI https://www.thermofisher.com/nl/en/home/electron- microscopy/products/software-em-3d-vis.html Deposited data Raw data are publicly available on FigShare 10.6084/m9.figshare.26219009 Genbank formatted maps of all plasmids are available on FigShare 10.6084/m9.figshare.26219009 Jo urn al Pr e-p roo f Plasmid Description Identifier pPL7428 PMP22-msGFP in pcDNA3. drug selection: Amp. pPL7428. .. Reagent or Resource Source Identifier Antibodies mouse α-GFP monoclonal Santa Cruz Technology, Santa Cruz, CA sc-9996 mouse α-HA monoclonal BioLegend, San Diego, CA cat# 901514 mouse α-myc-Tag (9B11) monoclonal Cell Signaling Technology, Danvers, MA cat# 2276 rabbit α-mCherry polyclonal EMD Millipore Corp., Burlington, MA AB356482 Peroxidase-conjugated (HRP) α- mouse Cell Signaling Technology cat#:70776S α-rabbit secondary antibody Cell Signaling Technology cat# 7074S α-GFP nanobody agarose Uiowa Protein crystallography facility Chicken α-GFP for immunogold labeling Abcam, Waltham, MA cat# ab13970 Rabbit α-mCherry for immunogold labeling Rockland Immunochemicals, Limerick PA cat#600-401-379 6 nm gold secondary donkey α- rabbit Jackson Immunoresearch, WestGrove, PA cat# 711-195-152 12 nm gold secondary donkey α- chicken Jackson Immunoresearch, WestGrove, PA cat# 703-205-155 Reagent or Chemical Dodecyl-β-D-maltoside (DDM) Anatrace (Maumee, OH) D310S cholesteryl hemisuccinate (CHS) Anatrace (Maumee, OH) CH210 Protease inhibitors cOmplete lacking EDTA Sigma (St. Louis, MO) Product COEDTAF-RO Pefabloc Sigma (St. Louis, MO) Product 11873601001 Dulbecco’s modified Eagle’s medium (DMEM) Gibco, Grand Island, NY REF: 11965-092 10% fetal bovine serum (FBS) Gibco, Grand Island, NY REF: 26140079 16% EM grade paraformaldehyde solution Electron Microscopy Sciences/ Fisher scientific REF: EMS 15710 Microscope sample carrier Leica, Wetzlar, Germany cat# 16770134 Dome-type carrier Leica, Wetzlar, Germany Software Fiji software (Image J) https://imagej.net/software/fiji/downloads Graphpad Prism https://www.graphpad.com/features FlowJo v10 https://www.flowjo.com T-coffee https://tcoffee.crg.eu/apps/tcoffee/index.html Photoshop https://www.adobe.com CS6 LAS X Microlab Leica, Wetzlar, Germany TIA software – Formerly Field Electron and Ion Company, FEI https://www.thermofisher.com/nl/en/home/electron- microscopy/products/software-em-3d-vis.html Deposited data Raw data are publicly available on FigShare 10.6084/m9.figshare.26219009 Genbank formatted maps of all plasmids are available on FigShare 10.6084/m9.figshare.26219009 Jo urn al Pr e-p roo f Plasmid Description Identifier pPL7428 PMP22-msGFP in pcDNA3.

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Article Title: Splicing variants in MYRF cause partial loss of function in the retinal pigment epithelium leading to nanophthalmos
Article Snippet: After 24 hours, cells were transduced with 1 × 10 –2 dilution of WT-MYRF, C-terminal mutant MYRF, or V679A (10X concentrated) vectors in the presence of 100 mg/mL LentiBoost (Mayflower Biosciences, St. Louis, MO, #SB-P-LV-101-11). .. Cells were fixed with 4% PFA (paraformaldehyde) in 1× PBS (phosphate buffered saline) for 10 min, incubated with rabbit anti-FLAG 1:1,000 (Cell Signaling Technology, Danvers, MA, #14793) and mouse anti-Calnexin 1:200 (Sigma Aldrich, Burlington, MA, #C4731) primary antibodies overnight at 4°C followed by incubation with anti-goat rabbit Alexa Fluor 488 1:500 (Jackson ImmunoResearch Labs, WestGrove, PA, #111-545-144) and goat anti-mouse Alexa Fluor 555 1:1,000 (Invitrogen, Carlsbad, CA, A-21422) secondary antibodies for 2 hours at room temperature (RT). .. Cells were washed 3× 5 min with 1×PBS,stained with DAPI (Thermo Fisher Scientific, Waltham,MA), mounted with gold anti-fade media (Thermo Fisher Scientific, Waltham, MA, P36930 ), and imaged at 63X using Leica SP8 confocal microscope (Leica, Wetzlar, Germany).

Article Title: Splicing variants in MYRF cause partial loss-of-function in the retinal pigment epithelium leading to nanophthalmos.
Article Snippet: After 24 hours, cells were transduced with 10-2 631 dilution of WT-MYRF, C-terminal mutant MYRF, or V679A (10X concentrated) vectors in 632 the presence of 100mg/mL LentiBoost (Mayflower Biosciences, St. Louis, MO, # SB-P-633 LV-101-11). .. Cells were fixed with 4% PFA (paraformaldehyde) in 1X PBS (phosphate 634 buffered saline) for 10 min, incubated with rabbit anti-FLAG 1:1000 (Cell Signaling 635 Technology, Danvers, MA, #14793) and mouse anti-Calnexin 1:200 (Sigma Aldrich, 636 Burlington, MA, #C4731) primary antibodies overnight at 4C followed by incubation with 637 anti-goat rabbit Alexa Fluor 488 1:500 (Jackson ImmunoResearch Labs, WestGrove, PA, 638 # 111-545-144) and goat anti-mouse Alexa Fluor 555 1:1000 (Invitrogen, Carlsbad, CA, 639 A-21422) secondary antibodies for 2 hours at RT. .. Cells were washed 3X 5 min with 640 1XPBS,stained with DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific, 641 Waltham,MA), mounted with gold anti-fade media (Thermo Fisher Scientific, Waltham, 642 MA, P36930), and imaged at 63X using Leica SP8 confocal microscope (Leica, Wetzlar, 643 Germany).

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Article Title: Anatomic and neurochemical analysis of the palpal olfactory system in the red flour beetle Tribolium castaneum , HERBST
Article Snippet: Cy 5 coupled goat anti-mouse , GAM-Cy 5 , Goat , 1:300 , Jackson ImmunoResearch; Westgrove, PA, USA (115-175-146, n/a, AB_2338713 ) , , .

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